Figure 5.

Translesion synthesis activities of POLN derivatives. Increasing amounts of delP, WT, K679R, K679T, K679A and K679Q (6, 12 and 23 nM), Y682F (6, 12, 23, 29, 58 and 115 nM), POLQ (3, 6 and 12 nM) and RB69 gp43 (2.5, 5 and 10 pM) were incubated with the 5′-³²P-labeled primer templates indicated, in the presence of all four dNTPs for 10 min. The first lane contained no enzyme. The first base templates were 5S-Tg (A), 5R-Tg (B) or AP analog (C). The percentage (%) extension of the primer is shown below each lane.