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. 2010 Feb 9;38(10):3233–3244. doi: 10.1093/nar/gkq048

Figure 6.

Figure 6.

Mutation of Lys 679 to Ala or Thr reduces strand displacement. Increasing amounts of delP, WT, K679R, K679T, K679A and K679Q (12, 23 and 46 nM), and Y682F (12, 23, 46, 58, 115 and 230 nM) and RB69 gp43 (5, 10, 20, 103, 205 and 410 pM) were incubated at 37°C for 15 min with 100 fmol primer annealed to M13mp18GTGx (A) or nicked substrate, 100 fmol primer and downstream oligomer were annealed to M13mp18GTGx (B). The substrates are schematically diagrammed and were radiolabeled at the 5′-end of the upstream 24-mer primer (asterisk). The 5′-end of the downstream 60-mer oligonucleotide was phosphorylated, and its 3′-end was blocked with ddATP. The first lane contained no enzyme. The percentage (%) extension of the primer is shown below each lane.