Table 2.
Summary of error rates for POLN and POLN derivatives
| WTa | K679Ta | K679Aa | ||||
|---|---|---|---|---|---|---|
| LacZ mutant frequency | 0.12 | 0.023 | 0.034 | |||
| Total mutants sequenced | 47 | 45 | 44 | |||
| Total bases sequenced | 19 129 | 18 315 | 17 908 | |||
| Detectable changesb | No. of events | Error rate (×10−4) | No. of events | Error rate (×10−4) | No. of events | Error Rate (×10−4) |
| Base substitutions | 54 | 18.0 | 28 | 1.9 | 43 | 4.4 |
| Frameshifts (−1) | 12 | 2.5 | 8 | 0.3 | 3 | 0.2 |
| Frameshifts (+1) | 2 | 1.3 | 5 | 0.2 | 1 | 0.1 |
| G:dTMP | 46 | 86.0 | 20 | 8.0 | 34 | 20.0 |
aFor WT, the total number of plaques scored was 6793, among which 789 were scored as mutants. For K679T the total number of plaques scored was 3380, among which 78 were scored as mutants. For K679A, the total number of plaques scored was 2552, among which 87 were scored as mutants.
bError rates were calculated for phenotypically detectable changes, as described in ‘Materials and Methods’ section.