Figure 1.

Schematic representation of RNP cDNA library generation and analysis. Left: RNP extracts were size-fractionated on glycerol gradients, with separation of RNPs in the size range from ∼10S to 30S. RNPs, consisting of ncRNAs and proteins, penetrate into the gradient, whereas short, non-functional RNA degradation products appear at the top of the gradient and are discarded; ribosomes and ribosomal subunits are pelleted at the bottom of the gradient. Each cDNA library corresponds to a distinct sedimentation range in the gradient (see text). Right: overview of sequence analysis of 96 clones from each cDNA library. From top to bottom, 94, 63, 68, 85, 66 and 89 clones out of 96 were exploitable, respectively. (Miscellaneous RNA: RNAse P RNA, 7SK RNA, Y RNA, 7SL RNA).