Table 2.
Additional cell line growth conditions and subtypes
| Lane | Name | Subtype* | Medium | Culture condition |
|---|---|---|---|---|
| 30 | 184A1N4 | N | MEGMa | 37°C, 5% CO2 |
| 31 | 184B5 | N | MEGMa | 37°C, 5% CO2 |
| 36 | HCC1599 | Basal A | RPMI1640+10%FBSb | 37°C, 5% CO2 |
| 48 | HCC1395 | Basal B | RPMI1640+10%FBS | 37°C, 5% CO2 |
| 50 | HCC1806 | Basal A | RPMI1640+10%FBS | 37°C, 5% CO2 |
| 53 | HCC2218 | Luminal | RPMI1640+10%FBS | 37°C, 5% CO2 |
| 54 | HCC1599 | Basal A | RPMI1640+10%FBS | 37°C, 5% CO2 |
| 55 | UACC893 | Luminal | DMEM+10% FBS | 37°C, 5% CO2 |
| 56 | SUM229PE | N/A | Ham's F12+5% FBS+IHc | 37°C, 5% CO2 |
| 57 | MX-1 | N/A | RPMI1640+10%FBS | 37°C, 5% CO2 |
| 58 | SUM102PT | Basal A | Ham's F12+IHEd | 37°C, 5% CO2 |
| 60 | HCC1419 | Luminal | RPMI1640+10%FBS | 37°C, 5% CO2 |
| 62 | S1 | N | H14 medium +10 ng/ml EGF | 37°C, 5% CO2 |
| 63 | T4 | Basal B | H14 mediume | 37°C, 5% CO2 |
| 64 | MDAMB231-Gray | Basal B | DMEM+10% FBS | 37°C, 5% CO2 |
| 65 | MDAMB231-ATCC | Basal B | DMEM+10% FBS | 37°C, 5% CO2 |
a: Clonetics MEBM (no Bi Carbonate)+Insulin(5 ug/ml)+Transferrin(5 ug/ml)+Hydrocortisone(0.5 ug/ml) +EGF(5 ng/ml) +Isoprorternol
b: Fetal bovine serum (FBS)
c: Ham's F12 + 5% FBS + IH {insulin (5 ug/ml) + Hydrocortisone (1 ug/ml) + HEPES (10 mM)}
d: Ham's F12 + IHE {insulin (5 ug/ml) + HEPES (10 mM) + EGF (10 ng/ml)}
e: H14 medium: DMEM/F12 (GIBCO/BRL) with 250 ng/ml insulin, 10 μg/ml transferrin, 2.6 ng/ml sodium selenite, 10-10 M estradiol, 1.4 × 10-6 M
*: N: non-malignant(immortalized), N/A: no data