Fig. 4.
LPS exposure leads to elevated ADMA and decreased DDAH activity in the mouse lung. ADMA levels and DDAH activity were analyzed by high-performance liquid chromatography (HPLC) in the mice lungs after LPS exposure. There was a progressive increase in ADMA levels after 2-, 4- and 12-h LPS exposure (A). Although there were no changes in either DDAH I (B) or DDAH II (C) protein levels there was a significant decrease in DDAH activity 2- and 4-h after LPS exposure (panel D). Values are mean ± SE, N=5 for each group, *P<0.05 compared to no LPS.