Table 2.
Reporter and detector oligonucleotides used in genotyping AtIAP1 segregating sites −361, −208, 542, 555 and 570.
| Oligonucleotide Name |
Sequence (5' to 3') | Optimal Ligation Temp (°C) |
|---|---|---|
| IAP-361dtcA | Biotin-TGATGATGGCRAYGATGCACT | 60 |
| IAP-361dtcC | Biotin-TGATGATGGCRAYGATGCACG | |
| IAP-361rpt | PO4-GAAAGATGCTGATGCTGT-Fluorescein | |
| IAP-208dtcA | Biotin-TCAATCTTGTTCTCCGGGGGT | 51 |
| IAP-208dtcC | Biotin-TCAATCTTGTTCTCCGGGGGG | |
| IAP-208rpt | PO4-TTTTCCGCRGTTCAGCTC-Fluorescein | |
| IAP542dtcC | Biotin-TTTCTGCTGWGGCAGCGCGGGCG | 58 |
| IAP542dtcT | Biotin-TTTCTGCTGWGGCAGCGCGGGCA | |
| IAP542rpt | PO4-CAGGAAGTTGTTGGGGGA-Fluorescein | |
| IAP555dtcA | Biotin-ATTCYGGCCGTTTCTGCTGT | 58 |
| IAP555dtcT | Biotin-ATTCYGGCCGTTTCTGCTGA | |
| IAP555rpt | PO4-GGCAGCGCGGGCRCAGGA-Fluorescein | |
| IAP570dtcA | Biotin-ATGGCATAGTTGGGATATTCT | 58 |
| IAP570dtcG | Biotin-ATGGCATAGTTGGGATATTCC | |
| IAP570rpt | PO4-GGCCGTTTCTGCTGWGGC-Fluorescein |