Table 1.
Parameters used in the kinetic model for the closed chamber apparatus and in the reaction-diffusion model for the open system
Parameter | Value | Reference |
---|---|---|
V | 8.8 × 10−3 L | see text |
v | 8.8 × 10−13 L | see text |
a | 1.77 × 10−10 m2 | see text |
ka | 2.6 × 106 M−2 s−1 | (11) |
km | 0.4 × 10−9 M s−1 | (19) |
KM | 7 × 10−6 M | (19) |
KI | 18 × 10−9 M | (19) |
DNO | 3.0 × 10−5 cm2 s−1 | (35) |
DO2 | 2.8 × 10−5 cm2 s−1 | (37) |
C0 | 223 µM | (36) |
Symbols: V, liquid volume in the closed chamber; v, volume of a single cell; a, area of a flat surface occupied by a single cell; ka, rate constant for autoxidation of NO; km, rate of NO consumption by media-generated O2−; KM, the O2 concentration at which respiration is halved in the absence of NO; KI, constant describing inhibition of respiration by NO; DNO and DO2, aqueous diffusivities of NO and O2 respectively; C0, liquid-phase O2 concentration in equilibrium with the incubator.