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. Author manuscript; available in PMC: 2011 Apr 19.
Published in final edited form as: Chem Res Toxicol. 2010 Apr 19;23(4):778–787. doi: 10.1021/tx900415k

Table 1.

Parameters used in the kinetic model for the closed chamber apparatus and in the reaction-diffusion model for the open system

Parameter Value Reference
V 8.8 × 10−3 L see text
v 8.8 × 10−13 L see text
a 1.77 × 10−10 m2 see text
ka 2.6 × 106 M−2 s−1 (11)
km 0.4 × 10−9 M s−1 (19)
KM 7 × 10−6 M (19)
KI 18 × 10−9 M (19)
DNO 3.0 × 10−5 cm2 s−1 (35)
DO2 2.8 × 10−5 cm2 s−1 (37)
C0 223 µM (36)

Symbols: V, liquid volume in the closed chamber; v, volume of a single cell; a, area of a flat surface occupied by a single cell; ka, rate constant for autoxidation of NO; km, rate of NO consumption by media-generated O2−; KM, the O2 concentration at which respiration is halved in the absence of NO; KI, constant describing inhibition of respiration by NO; DNO and DO2, aqueous diffusivities of NO and O2 respectively; C0, liquid-phase O2 concentration in equilibrium with the incubator.