. Author manuscript; available in PMC: 2011 Apr 19.

Published in final edited form as: Chem Res Toxicol. 2010 Apr 19;23(4):778–787. doi: 10.1021/tx900415k

Table 2.

Cellular kinetic parameters for NO, O₂, and O₂⁻ measured in the closed-chamber experiments.

Kinetic Parameter	RAW264.7	BMDM	HCT116
R_max^o (pmol s⁻¹ 10⁶ cells⁻¹)	38 ± 4 (n = 4)	30 ± 3 (n = 4)	26 ± 7 (n = 4)^†
R_max (pmol s⁻¹ 10⁶ cells⁻¹)	62 ± 14 (n = 6)	59 ± 17 (n = 6)	41 ± 11 (n = 6) ^‡
k_g (pmol s⁻¹ 10⁶ cells⁻¹)	2.1 ± 0.3 (n = 6)	2.3 ± 0.6 (n = 6)	1.1 ± 0.2 (n = 6) ^†^‡
k_c (s⁻¹)	1.6 ± 0.4 (n = 6)	1.3 ± 0.3 (n = 6)	4.1 ± 2.3 (n = 6) ^†^‡
$k_{s}^{o}$ (pmol s⁻¹ 10⁶ cells⁻¹)	0.07 ± 0.02 (n = 4)	0.05 ± 0.01(n = 4)	-
k_s (pmol s⁻¹ 10⁶ cells⁻¹)	0.3 ± 0.09 (n = 4)	0.3 ± 0.08 (n = 4)	-

Symbols: R_max^o and R_max, maximum respiration rates for unactivated and activated cells, respectively; k_g, rate of generation of NO; k_c, rate constant for intracellular consumption of NO for activated cells; k_s^o and k_s, rates of extracellular O₂⁻ generation by unactivated and activated macrophages, respectively. All values are mean ± SD for the number of experiments indicated.

^†

p < 0.05 versus RAW264.7 values,

^†

p < 0.05 versus BMDM values.