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. Author manuscript; available in PMC: 2011 Apr 19.
Published in final edited form as: Chem Res Toxicol. 2010 Apr 19;23(4):778–787. doi: 10.1021/tx900415k

Table 3.

Conditions in the open-system experiments and comparative rates of NO synthesis and loss to the headspace.

Parameter RAW264.7 BMDM HCT116 RAW264.7
Stimulant IFNγ + LPS IFNγ + LPS TNF-α + IFN-γ,
H.Cinaedi
H.Cinaedi
α 0.35 0.2 0.2 0.2
β 0.15 0.15 0 0.15
L 5 mm 5 mm 2 mm 2 mm
Fraction NO lost 0.32 0.43 0.82 0.71
kg . pmol s−1 (106 cells)−1) 2.5 ± 0.6

(n = 2)
2.3 ± 0.9

(n = 2)
3.9 ± 0.1

(n = 5)
2.6 ± 0.1

(n = 5)

Symbols: α, fraction of plate area covered by cells; β, ratio of O2 to NO flux at cells; L, liquid depth; kg, rate of NO generation by cells. Values for kg are mean ± SD.