Table 3.
Conditions in the open-system experiments and comparative rates of NO synthesis and loss to the headspace.
| Parameter | RAW264.7 | BMDM | HCT116 | RAW264.7 |
|---|---|---|---|---|
| Stimulant | IFNγ + LPS | IFNγ + LPS | TNF-α + IFN-γ, H.Cinaedi |
H.Cinaedi |
| α | 0.35 | 0.2 | 0.2 | 0.2 |
| β | 0.15 | 0.15 | 0 | 0.15 |
| L | 5 mm | 5 mm | 2 mm | 2 mm |
| Fraction NO lost | 0.32 | 0.43 | 0.82 | 0.71 |
| kg . pmol s−1 (106 cells)−1) | 2.5 ± 0.6 (n = 2) |
2.3 ± 0.9 (n = 2) |
3.9 ± 0.1 (n = 5) |
2.6 ± 0.1 (n = 5) |
Symbols: α, fraction of plate area covered by cells; β, ratio of O2− to NO flux at cells; L, liquid depth; kg, rate of NO generation by cells. Values for kg are mean ± SD.