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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Cell Biochem. 2010 Jun 1;110(3):645–659. doi: 10.1002/jcb.22575

Figure 1. Carboxylated glycans on RAGE mediate binding to S100A12, but not to S100A11.

Figure 1

To determine binding equilibrium of purified human S100A12 (A) or S100A11 (B) to purified bovine RAGE, increasing amounts of the ligands were added to total RAGE, mAbGB3.1 enriched RAGE, or RAGE deglycosylated using PNGaseF under non-denaturing conditions that removed both N-glycans. RAGE on plate was quantified using anti-RAGE. Bound ligands were quantified using specific antibodies. Data were fitted to non-linear regression analysis using GraphPad Prism. Each point is the mean ± SD of two determinations.