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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Cell Biochem. 2010 Jun 1;110(3):645–659. doi: 10.1002/jcb.22575

Figure 2. S100A12 shows specific binding to carboxylated glycans.

Figure 2

Purified recombinant human S100A12 (A) was incubated with BSA-conjugated carboxylate-enriched bovine lung glycopeptides coated onto microtiter plates and binding was detected using anti-S100A12. Non-linear regression transforms of binding data using GraphPad Prism are presented. (B) Inhibitions of binding were carried out in presence of increasing concentrations of free carboxylated or carboxylate-neutralized glycans. Each point is the mean two determinations. C. Time course of complexation between S100A12 and carboxylated glycans (0.1M, moderately charged, 0.3M highly charged) in the presence or absence of calcium was measured by light scattering at 420 nm.