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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Cell Biochem. 2010 Jun 1;110(3):645–659. doi: 10.1002/jcb.22575

Figure 3. Glycosylation of sRAGEs expressed in different cells.

Figure 3

A. sRAGEs were expressed in different mammalian cells using retroviral vectors and in SF9 cells and purified as described under Methods. Purity was confirmed by SDS-PAGE and Coomassie brilliant blue staining. Western blots show that sRAGEs from different sources are all glycosylated. Lane 1: undigested. Lane 2: Endo H digested Lane 3: PNGase F digested. B. mAbGB3.1 reactivity of the different preparations as determined by ELISA. C. There is direct correlation between mAbGB3.1 reactivity of the human sRAGEs and bovine RAGE, and their maximum binding sites for human S100A12.