Skip to main content
. 2010 Apr 16;22(4):1358–1372. doi: 10.1105/tpc.109.072058

Figure 4.

Figure 4.

Analysis of the Effect of Nt-RDR1 on S-PTGS and Secondary siRNA Synthesis.

(A) and (B) Induction of silencing of the GFP transgene in 16c, 16c/NtRDR1, and 16c/RDR6i plants. (A) shows an RNA gel blot analysis of G-, F-, and P-derived siRNA accumulation in 16c, 16c/NtRDR1, and 16c/RDR6i plants infiltrated with 35S-Fi or 35S-F at 4 dpa. 32P-labeled RNA probes specific for each corresponding GFP portion were used. U6 RNA hybridization is shown as loading control. (B) shows the systemic spread to the whole plant of GFP silencing induced by transient expression of 35S-Fi in 16c and 16c/NtRDR1 plants and the limited silencing spread to one or two upper leaves in a 16c/RDR6i plant. Photographs were taken under UV light at 2 weeks after agroinfiltration.

(C) and (D) Induction of silencing of transiently expressed GFP in Nb, Nt-RDR1, and RDR6i plants. (C) shows an RNA gel blot analysis of G-, F-, and P-derived siRNA accumulation in plants coinfiltrated with 35S-GFP and 35S-Fi or 35S-F as described in (A). (D) shows leaves of Nb, Nt-RDR1, and RDR6i plants coinfiltrated with 35S-GFP and 35S-F. Photographs were taken under UV light at 4 dpa.