Table 1.

Genetic Analysis of Transmission Efficiency of ahp1,2-1,3,4,5 and ahp1,2-2,3,4,5

Parent		F1 Genotype
Female	Male	AHP/AHP	AHP/ahp	T-DNA TE (%)
Col-0	ahp1,2-1,3/+,4,5	51	42	45.16
Col-0	ahp1,2-1,3,4,5/+	31	36	53.73
Col-0	ahp1,2-2,3/+,4,5	47	38	44.71
Col-0	ahp1,2-2,3,4,5/+	47	47	50.00
ahp1,2-1,3/+,4,5	Col-0	83	69	45.39
ahp1,2-1,3,4,5/+	Col-0	83	72	46.45
ahp1,2-2,3/+,4,5	Col-0	196	7	3.45
ahp1,2-2,3,4,5/+	Col-0	93	1	1.06

The ahp mutants used in crosses were heterozygous for the T-DNA insertion at AHP3 (ahp1,2,3/+,4,5) or AHP5 (ahp1,2,3,4,5/+) and were homozygous at other loci. Genotypes of both parents and the resulting progenies were determined by PCR analysis as described in Methods. Success of crossing was confirmed by genotyping F1 progenies using appropriate simple sequence length polymorphism markers (approximately three to five markers were used in each assay). Transmission efficiency (TE) is calculated as 100 × heterozygous/(heterozygous + wild type).