Table 2.
Genotypea | Constructb | Testedc | cki1-5/−d | Percentage |
cki1-5/+ | – | >500 | 0 | 0.00 |
cki1-5/+ | Empty vector | 159 | 0 | 0.00 |
cki1-5/+ | ARR1 | 100 | 7 | 7.00 |
cki1-5/+ | ARR1ΔDDK | 201 | 18 | 8.96 |
cki1-5/+ | ARR1D94E | 47 | 4 | 8.51 |
cki1-5/+ | ARR1D94N | 233 | 15 | 6.44 |
cki1-5/+ | IPT8 | 58 | 2 | 3.45 |
Heterozygous cki1-5/+ plants were transformed with different constructs.
All transgenes were placed under the control of the CKI1 promoter and were cloned into the binary vector pER8 (Zuo et al., 2000) that carried a hygromycin selectable marker gene. “Empty vector” refers to the use of pER8 in the transformation. “–” Denotes self-pollinated nontransgenic plants.
“Tested” refers to the tested T1 transgenic lines that were resistant to hygromycin and carried a cki1-5 mutation. For nontransgenic plants, the number refers to the analyzed progeny obtained from self-pollinated cki1-5/+ plants that carried a cki1-5 mutation.
“cki1-5/−” refers to homozygous lines (transgenics) or families (self-pollinated) that were obtained from the tested populations by PCR genotyping and phenotypic analysis.