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. 2010 Apr 2;22(4):1232–1248. doi: 10.1105/tpc.108.065128

Table 2.

Rescue of the cki1-5 Mutant Phenotype by ARR1 and IPT8 Transgenes

Genotypea Constructb Testedc cki1-5/−d Percentage
cki1-5/+ >500 0 0.00
cki1-5/+ Empty vector 159 0 0.00
cki1-5/+ ARR1 100 7 7.00
cki1-5/+ ARR1ΔDDK 201 18 8.96
cki1-5/+ ARR1D94E 47 4 8.51
cki1-5/+ ARR1D94N 233 15 6.44
cki1-5/+ IPT8 58 2 3.45
a

Heterozygous cki1-5/+ plants were transformed with different constructs.

b

All transgenes were placed under the control of the CKI1 promoter and were cloned into the binary vector pER8 (Zuo et al., 2000) that carried a hygromycin selectable marker gene. “Empty vector” refers to the use of pER8 in the transformation. “–” Denotes self-pollinated nontransgenic plants.

c

“Tested” refers to the tested T1 transgenic lines that were resistant to hygromycin and carried a cki1-5 mutation. For nontransgenic plants, the number refers to the analyzed progeny obtained from self-pollinated cki1-5/+ plants that carried a cki1-5 mutation.

d

cki1-5/−” refers to homozygous lines (transgenics) or families (self-pollinated) that were obtained from the tested populations by PCR genotyping and phenotypic analysis.