Figure 9.

The j3-1 pks5 Double Mutants Phenocopy the pks5 Response to Salt in Alkaline Conditions.

(A) to (I) Five-day-old j3-1, pks5-1, j3-1 pks5-1, pks5-3, j3-1 pks5-3, pks5-4, and j3-1 pks5-4 mutant seedlings grown on MS medium at pH 5.8, at pH 7.7 with 75 mM NaCl, or at pH 8.1 with 75 mM NaCl. Photographs in (A), (D), and (G) were taken 7 d after transfer; in (B), (E), and (H), 14 d after transfer; and in (C), (F), and (I), 21 d after transfer.

(J) Primary root elongation of plants transferred to MS medium at pH 5.8.

(K) Primary root elongation of plants transferred to MS medium at pH 7.7 with 75 mM NaCl.

(L) Primary root elongation of plants transferred to MS medium at pH 8.1 with 75 mM NaCl.

In (J) to (L), primary root length was measured 7, 14, and 21 d after transfer, respectively. Error bars represent sd (plant number >15). A Student's t test was used to determine statistical significance; significant differences (P ≤ 0.05) in (J) to (L) are indicated by different lowercase letters.

[See online article for color version of this figure.]