Figure 2.

Rhizogenes Transformation and Haustorium Phenotypes.

Photos in (A), (B), (D), (F), (G), (H), (J), (L), and (N) are bright-field images. Photos in (C), (E), (I), (K), (M), and (O) are epifluorescence images showing YFP expression. Bars = 1 cm for (A) and (B) and 1 mm for (C) to (G).

(A) Triphysaria seedlings on day 0 of transformation.

(B) A branched Triphysaria root 12 weeks after transformation. Six weeks prior to this photograph, a root tip was cut to stimulate new root development at or near the cut site. Root tips were cut a second time resulting in the highly branched transgenic root seen in (C).

(C) A magnification of a section of (B) showing a cluster of YFP expressing roots, all derived from a single transformation event.

(D) and (E) Transgenic roots formed multiple haustoria as marked by arrowheads.

(F) A completely developed haustorium on a pHG8-YFP root.

(G) A partially developed haustorium on a pHpQR1 root.

(H) and (I) A nontransgenic and pHG8-YFP transgenic root side by side, both with a haustorium. The arrow points to the nontransgenic root.

(J) and (K) Haustorium developing on a pHG8-YFP transgenic root formed in response to an Arabidopsis host root (arrowhead).

(L) and (M) A transgenic haustorium 24 h after initiation. There is little YFP expression in the newly formed haustorium.

(N) and (O) A transgenic haustorium 48 h after initiation. YFP expression within the haustorium is similar to other regions of the root.

[See online article for color version of this figure.]