Table 2.

Haustorium Development in Transgenic Roots

	Percentage of Roots with Haustoriaa
Treatment	Seedlingsb	pHG8-YFP	pHpQR2	pHpQR1	pHpQR2-QR1
Host rootsc	87 ± 9	84 ± 11 *	80 ± 10*	28 ± 6**	49 ± 4***
	n = 90	n = 113	n = 100	n = 112	n = 103
Host root exudatesd	85 ± 3	81 ± 11 *	87 ± 9*	28 ± 12**	40 ± 8***
	n = 214	n = 118	n = 145	n = 98	n = 131
DMBQe	82 ± 5	87 ± 15*	87 ± 9*	28 ± 7**	48 ± 9***
	n = 186	n = 113	n = 129	n = 123	n = 69
Syringic acidf	82 ± 4	81 ± 7*	81 ± 11*	25 ± 4**	50 ± 11***
	n = 239	n = 145	n = 126	n = 124	n = 130
2-Methyl benzoquinoneg	45 ± 4	45 ± 8*	43 ± 5*	12 ± 4**	24 ± 7***
	n = 239	n = 114	n = 127	n = 119	n = 129
Peonidinh	71 ± 10	64 ± 3*	67 ± 9*	27 ± 9**	44 ± 6***
	n = 196	n = 155	n = 109	n = 118	n = 106
Water	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0
	n = 186	n = 104	n = 97	n = 102	n = 117

^aValues are means ±sd of 3 to 11 plates with four to six independently transformed plants with 1 to 30 roots (n = total number of roots in all replicates). Analysis of variance was determined with the General Linear Model procedure and mean separation with the least significance difference method using Statistical Analysis Software 9.1 (SAS Institute). Pairwise comparisons of treatments within a row labeled with a different number of asterisks are significantly different at α = 0.05. Haustoria were counted 24 h after treatment except with host roots.

^bSeedling data were not included in statistical analysis because they had different ages.

^cTriphysaria roots were placed in contact with Arabidopsis roots for 5 d.

^dMedicago host exudates were obtained as described in Methods.

^eThe 30 μM 2,6-DMBQ was from Pfalz and Bauer.

^fThe 1000 μM syringic acid was from Sigma-Aldrich.

^gThe100 μM 2-MBQ was from Sigma-Aldrich.

^hThe30 μM peonidin chloride was from Indofine Chemical.