Figure 6.

Effects of Exogenous dCel1p and Purified Anti-dCel1p Antibody on Cell Cycle Progression.

(A) The addition of exogenous dCel1p to the cells at T = 7 (early G₂) advanced the cell cycle. In the presence of exogenous dCel1p, the cells completed the cell cycle and returned to the G₁ phase earlier (as shown by the emergence of first peak at T = 10 in the flow cytogram; indicated by the arrow) than in the controls. An equal amount of boiled recombinant dCel1p was added to the culture as control.

(B) Cells treated with exogenously added dCel1p were generally larger in size (at T = 8, G₂/M phase) than the control cells.

(C) Purified anti-dCel1p antibody suppressed the activity of recombinant dCel1p. Recombinant dCel1p was incubated with purified anti-dCel1p antibody for 1 h at 4°C prior to cellulase activity assay. Data represent means ± se of four replicate experiments. Asterisk indicates significant (P < 0.01) difference compared with the control.

(D) The addition of affinity-purified anti-dCel1p antibody to the cells at T = 7 (early G₂) resulted in a G₂/M delay. Most of the cells treated with the affinity-purified antibody were still in the G₂/M phase (the second peak at T = 12 was higher than the first peak, indicated by the arrow) at T = 12. An equal amount of boiled antibody was added to the culture as a control.

(E) Cells treated with the anti-dCel1p antibody were generally smaller (at T = 8, G₂/M phase) than the control cells.

[See online article for color version of this figure.]