Figure 6.

TCP1 Positively Regulate the Expression of DWF4.

(A) Real-time RT-PCR results indicate that the expression of DWF4 is elevated in tcp1-1D but reduced in TCP1-SRDX plants. DWF4 expression level in WS2 was set to 1. DWF4 expression levels in other genotypes were relative to that in WS2. The data were the average of three replicates. The error bars represent the sd.

(B) The top panel is the immunoblotting data indicating that tcp1-1D positively, whereas TCP1-SRDX negatively regulates the expression of DWF4p-GUS. The bottom panel is a duplicate polyacrylamide gel stained with Coomassie blue to show equal loading.

(C) tcp1-1D does not rescue the phenotypes of a null dwf4 mutant. The plants were photographed 4 weeks after germination.

[See online article for color version of this figure.]