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Suspension cell culture alkalinization assay. Change in pH was measured in cell suspension culture 15 min after exposure to unaltered or alkalinized vegetative (SlRALF and alkSlRALF) and pollen (SlPRALF and alkSlPRALF) RALF peptides at 0.25 nm (white bars), 2.5 nm (light gray bars), 25 nm (dark gray bars), and 250 nm (black bars). A, S. peruvianum suspension cell culture assay. B, Arabidopsis suspension cell culture assay.
