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. 2010 Apr 13;153(2):703–715. doi: 10.1104/pp.110.155457

Figure 8.

Figure 8.

Reversibility of SlPRALF inhibition. A, Histogram of percentage of hydrated pollen with TGR ± se 1 h after time-limited treatment with 0.1 μm SlPRALF. Pollen was treated with peptide for designated exposure times, pelleted by centrifugation, and resuspended in medium containing 0.1 μm SlPRALF (constant SlPRALF, spin = black bars) or no peptide (SlPRALF removed, spin = dark gray bars). Samples not treated with peptide were analyzed without centrifugation (no SlPRALF, no spin = white bars) or with centrifugation (no SlPRALF, spin = light gray bars). n ≥ 100. B, Micrograph of pollen in peptide-removed PGM 1 h after 15 min of SlPRALF treatment. C, Micrograph of pollen in peptide-removed PGM 1 h after 20 min of SlPRALF treatment.