Figure 5.

Map-based cloning of OsMADS34. A, Fine mapping of the osmads34-1 on chromosome 3. Names and positions of the molecular markers are indicated. Numbers represent recombinant events. cM, Centimorgan. A total of 1,738 individuals of the F2 mapping population generated by a cross between osmads34-1 and cv Guang-lu-ai 4 (var indica) were analyzed using a set of primers (Supplemental Table S1). Finally, this mutant gene was located within a DNA region of 133 kb. B, A schematic representation of the exon and intron organization of OsMADS34. The mutant sequence has three nucleotide substitutions in the fifth intron. Black boxes indicate exons, and white boxes indicate introns. C, The OsMADS34 cDNA and protein sequences. The mutated protein sequence is underlined by the thick line. The MADS box domain is boxed. The K domain is marked by two lines, and the C domain is shaded. The black triangle marks the extra G in the osmads34-1 mutant cDNA. WT, Wild type.