Figure 5.

Composition of wild-type (WT) and cesa9 seed cell walls. A, Acid-insoluble crystalline cellulose content of various tissues was determined colorometrically for mature seed, leaves, and stems. Error bars are ses of three technical replicates from three independent batches of seed as biological replicates. CW, Cell wall. B, Calcofluor staining and subsequent illumination with UV light (scale bar = 250 μm). C, GC analysis of cell wall neutral sugars. Error bars are se of three replicates. D, Mucilage weights determined for wild-type and cesa9 seed, error bars are se of three replicates. E, Ruthenium red-stained seed visualized by light microscopy (scale bar = 200 μm). Col-0, Columbia-0. [See online article for color version of this figure.]