Table II. Enzyme activity in the apoplast of the tobacco cells at 5 d after onset of culture.
Fraction | Tobacco Cells | α-Xylosidase | β-Glucosidase | β-Xylosidase | PAP | Protein |
10−3 units per flask | μg per flask | |||||
Wall | Wild type | 73.9 ± 4.7 | 123 ± 11.7 | n.d. | 0.8 ± 0.1 | 56 |
Transgenic | 8.5 ± 1.3 | 82 ± 5.1 | n.d. | 16.5 ± 1.9 | 55 | |
Medium | Wild type | 39.9 ± 6.3 | 41 ± 3.8 | 29.9 ± 1.9 | n.d. | 48 |
Transgenic | 4.8 ± 0.5 | 8 ± 0.7 | 9.2 ± 1.0 | n.d. | 43 |