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Trx-dependent activation kinetics of ZmGLYK. Recombinant ZmGLYK (0.14 μm) was incubated at 23°C in 100 mm Tricine-KOH, pH 7.7, with 10 μm of different maize and E. coli Trxs plus 10 mm DTT or with DTT alone as a control. Aliquots were assayed for time-dependent activation.
