Figure 2.

AtHSBP gene structure and characterization of AtHSBP-knockout and -overexpression lines. A, AtHSBP gene is composed of four introns and five exons (black boxes). The 5′ and 3′ untranslated regions are indicated by hatched boxes. The T-DNA insertions are in the first intron (Athsbp-1, SALK_081104) and fourth intron (Athsbp-2, SALK_046465), as indicated by triangles. The specific primers for genotyping are shown by black arrows. ATG and TAA are shown as initiation and stop codons, respectively. B, Expression of AtHSBP in AtHSBP-knockout (Athsbp-1 and -2) and AtHSBP-overexpression (AtHSBP-ox1 and -ox2) lines was analyzed by northern-blot analysis (top; full-length AtHSBP cDNA as probe) and western-blot analysis (bottom; α-HA or α-RPN8 antibody). RPN8 and Rubisco large subunit stained with Amido black are shown for equal loading. WT, Wild type.