Table II. Basal and acquired thermotolerance tested in AtHSBP mutant lines.

Wild-type, AtHSBP-knockout (Athsbp-1 and -2), AtHSBP-overexpression (AtHSBP-ox1 and -ox2), and AtHSP101-knockout (Athsp101) lines were assayed for basal and acquired thermotolerance during seed germination, hypocotyl elongation, and seedling survival. Thermotolerance tests were calculated from 150 seedlings in each experiment. Data are means ± sd (n = 3). *, Significant at P < 0.05 (Student's t test, as compared with the wild-type value).

Line	Basal Thermotolerance Test		Acquired Thermotolerance Test
	Seed Germination, 45°C, 220 min	2.5-d-Old Hypocotyl Elongation, 44°C, 20 min	2.5-d-Old Hypocotyl Elongation, 37°C, 60 min, Recovery, 120 min, 44°C, 190 min	3-d-Old Seedling Survival, 37°C, 60 min, Recovery, 120 min, 44°C, 190 min	6-d-Old Seedling Survival, 37°C, 60 min, Recovery, 120 min, 44°C, 190 min
	% germination	% unheated growth	% wild-type survival
Wild type	99.2 ± 0.8	49.7 ± 3.9	25.3 ± 0.4	100	100
Athsbp-1	98.0 ± 1.8	45.7 ± 6.0	30.9 ± 1.0*	148.8 ± 10.7*	145.9 ± 9.6*
Athsbp-2	91.8 ± 4.7	49.1 ± 4.3	26.1 ± 0.7	156.4 ± 11.9*	149.0 ± 10.3*
AtHSBP-ox1	97.0 ± 0.9	52.3 ± 3.1	25.2 ± 0.4	84.0 ± 10.2*	83.5 ± 11.7*
AtHSBP-ox2	98.6 ± 1.3	46.2 ± 6.5	16.2 ± 0.1*	76.2 ± 7.2*	78.6 ± 11.1*
Athsp101	0	26.3 ± 4.3*	5.34 ± 0.7*	0*	0*