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. 2010 Apr 13;153(2):632–641. doi: 10.1104/pp.110.155796

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Figure 5. — CaLCuV-based expression of Arabidopsis miR156b and miR165a in N. benthamiana plants. A, Plant phenotypes associated with CaLCuV, CaLCuV-miR156, CaLCuV-miR156-m, CaLCuV-miR165, and CaLCuV-miR165-m at 10 dpi (top row) or 16 dpi (bottom row). B, End-point stem-loop RT-PCR (22 cycles) analysis showing the levels of mature miR156 and miR165 in inoculated plants. C, Semiquantitative RT-PCR analysis of the effects of CaLCuV-based expression of Arabidopsis miR156b and miR165a on their cognate target genes. Lanes 1 to 5 represent PCRs with 18, 21, 24, 27, and 30 cycles, respectively. PCR products to the left of the marker (M) are from the silenced plants, and those to the right are the nonsilenced controls. Actin mRNA was used as an internal control, and a no-RT control (C) was also included.