Figure 7.

Interactions between ABF and DREB family members. A, Two-hybrid assays were carried out to investigate the interactions between ABF and DREB family members. DREB2C, DREB2A, and DREB1A indicate prey constructs carried on pYesTrp-2. Lamin, ABF2, ABF3, and ABF4 denote bait constructs. Yeast containing each bait construct was transformed with individual prey constructs, transformants were grown on Gal/raffinose-His-Leu-Trp-Ura medium, and colony lift β-galactosidase assays were performed. B, In vitro interactions between ABF2 and DREBs. ABF2 interaction with DREB1A or DREB2A was investigated by GST pulldown assy. In vitro-translated DREB1A or DREB2A, each labeled with [³⁵S]Met, was allowed to bind GST or GST-ABF2 fusion protein, and bound protein was visualized by autoradiography after SDS-PAGE. The numbers indicate protein size in kD. [See online article for color version of this figure.]