Table 4.
FISH (% positive)
| Histologic Group |
Immunohistochemistry (% Positive) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Abnormality | Number of Patients (BP/MP/UC) | Biphasic | Monophasic | Unclassifiable | CD5 | CD23 | CD43 | IRF4/MUM1 | IgD | Typical SMZL Phenotype* |
| del(7)(q31) (n=8) | 15/12/16 | 20 | 33 | 6# | 13 | 13 | 25 | 38 | 88 | 50 |
| +18 (n=5) | 14/12/16 | 7 | 17 | 13 | 0 | 20 | 0 | 60 | 60 | 40 |
| +12 (n=14) | 12/12/16 | 17 | 33 | 50 | 7 | 0 | 21 | 29 | 62 | 38 |
| +5 (n=3) | 13/10/15 | 8 | 10 | 7 | 0 | 0 | 0 | 0 | 33 | 33 |
| +X (n=7) | 13/11/16 | 23 | 9 | 19 | 29 | 14 | 14 | 29 | 67 | 33 |
| +3q27 (n=11) | 13/9/14 | 31 | 22 | 36 | 20 | 18 | 10 | 45 | 91 | 60 |
| CDK6-R (n=2) | 11/10/15 | 0 | 0 | 13 | 0 | 0 | 50 | 0 | 50 | 0 |
| BCL3-R (n=1) | 12/10/15 | 0 | 0 | 7 | 0 | 0 | NE | 0 | 0 | NE |
| BCL2-R (n=0) | 11/10/15 | 0 | 0 | 0 | - | - | - | - | - | - |
| MALT1-R (n=0) | 14/11/16 | 0 | 0 | 0 | - | - | - | - | - | - |
| BCL6-R (n=2) | 13/9/14 | 8 | 11 | 0 | 50 | 0 | 0 | 50 | 100 | 50 |
BP = biphasic, MP = monophasic, UC = unclassifiable
R = Rearrangement
#
This represented one case that had three signals for the centromere of chromosome 7 and two signals for 7q31.
*
Typical SMZL phenotype considered to be CD5−, CD23−, CD10−, CD43−, and IgD+.
NE = Not evaluable
The following cutoffs were used for FISH positivity: 15% for del(7)(q31), BCL6-R, +3(q27), BCL3-R, +18, +12, +5, and +X; 20% for CDK6-R and MALT1-R; 30% for BCL2-R.