Fig. 2.

Hoechst 33347 staining. (A) After INS-1 cell were incubated under control, IHG and SHG, they were stained with Hoechst 33347 to detect apoptosis morphologically. The red arrows represent cells with DNA condensation. (B) The numbers of cells with broken DNA per 300 cells in three different fields in each condition of one experiment were counted and repeated in three different experiments. The mean percentages of positive staining under control, IHG and SHG were presented by histogram. C, control; IHG, intermittent high glucose; SHG, sustained high glucose. ^aP < 0.05 vs. control, ^bP < 0.05 vs. IHG.