TABLE 4.
Comparison of rates of oxygenation and product profiles for 5 μM AA, EPA, and DHA with native huPGHS-2 and aspirin-acetylated huPGHS-2
Rates and products were determined as described in Table 2 using 5 μM fatty acid substrate and 25 μM palmitic acid (16:0).
| Fatty Acids | huPGHS-2 |
ASA-huPGHS-2 |
||
|---|---|---|---|---|
| Rate | Products | Rate | Products | |
| μM FA/min/mg | % | μM FA/min/mg | % | |
| AA | 9.3 ± 0.51 | 15-HETE (6.8) | 5.9 ± 0.43 | 15-HETE (52) |
| 11-HETE (9.2) | 11-HETE (9.0) | |||
| PGH2 (84) | PGH2 (39) | |||
| AA + 16:0 | 16 ± 1.0 | 15-HETE (7.3) | 6.4 ± 0.51 | 15-HETE (27) |
| 11-HETE (9.6) | 11-HETE (4.1) | |||
| PGH2 (83) | PGH2 (69) | |||
| EPA | 4.1 ± 0.03 | 14-HEPE (15) | 1.5 ± 0.07 | 18-HEPE (2.2) |
| 15-HEPE (1.9) | 14-HEPE (16) | |||
| 11-HEPE (22) | 15-HEPE (16) | |||
| PGH3 (61) | 11-HEPE (18) | |||
| PGH3 (47) | ||||
| EPA + 16:0 | 4.3 ± 0.14 | 14-HEPE (14) | 1.5 ± 0.18 | 18-HEPE (2.0) |
| 15-HEPE (3.3) | 14-HEPE (12) | |||
| 11- HEPE (18) | 15-HEPE (23) | |||
| PGH3 (65) | 11-HEPE (18) | |||
| PGH3 (45) | ||||
| DHA | 2.0 ± 0.08 | 13-HDHA (100) | 1.1 ± 0.23 | 17-HDHA (78) |
| 13-HDHA (22) | ||||
| DHA + 16:0 | 2.0 ± 0.11 | 13-HDHA (100) | 1.1 ± 0.04 | 17-HDHA (73) |
| 13-HDHA (27) | ||||