Fig. 3.

Comparison of binding analyses using [³H]IP₃ and FP. FP experiment at 4°C using 0.5 nM FITC-IP₃ and showing corrected A as a function of increasing concentration of NT (A). Equilibrium competition binding experiments with [³H]IP₃ (0.75 nM), NT (4 μg), and the indicated concentrations of FITC-IP₃ (B). FP competition binding assay with FITC-IP₃ (0.5 nM), NT (80 nM), and the indicated concentrations of IP₃ (C) or adenophostin A (D). Results (A–D) are means ± S.E.M., n = 3. All binding analyses (A–D) were performed in CLM. Equivalent analyses with the IBC are shown in Supplemental Fig. 5.