Comparison of binding analyses using [3H]IP3 and FP.
FP experiment at 4°C using 0.5 nM FITC-IP3 and
showing corrected A as a function of increasing
concentration of NT (A). Equilibrium competition binding experiments with
[3H]IP3 (0.75 nM), NT (4 μg), and the
indicated concentrations of FITC-IP3 (B). FP competition binding
assay with FITC-IP3 (0.5 nM), NT (80 nM), and the indicated
concentrations of IP3 (C) or adenophostin A (D). Results
(A–D) are means ± S.E.M., n = 3.
All binding analyses (A–D) were performed in CLM. Equivalent
analyses with the IBC are shown in Supplemental Fig. 5.