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. 2010 Jun;77(6):995–1004. doi: 10.1124/mol.109.062596

TABLE 1.

Comparison of KD determined by [3H]IP3 binding and FP assays

The KD for each ligand was determined in CLM at 4°C either by competition binding with [3H]IP3 or by FP. Results are means ± S.E.M., n = 3.

IBC
NT
[3H]IP3 FP [3H]IP3 FP
nM
FITC-IP3 2.0 ± 0.2 3.0 ± 0.1 11.8 ± 0.2 12.5 ± 0.6
IP3 8.7 ± 1.8 9.2 ± 0.8 65.2 ± 8.2 94.8 ± 7.1
Adenophostin A 0.7 ± 0.1 0.9 ± 0.1 6.2 ± 1.6 8.6 ± 0.2