TABLE 3.

Temperature dependence of the K_D for agonist binding to the NT and IBC

FP was used to measure the K_D for each ligand for the IBC and NT at the indicated temperatures in CLM. Results are means ± S.E.M., n = 3.

	K D
	277 K	283 K	289 K	296 K	303 K	310 K
	nM
IP3
IBC	9 ± 0.6	12.6 ± 1.2	18.4 ± 2.2	23.5 ± 2.0	31.3 ± 2.2	52.9 ± 4.4
NT	95 ± 7	119 ± 10	191 ± 23	281 ± 23	377 ± 38	490 ± 40
Adenophostin A
IBC	0.9 ± 0.1	1.3 ± 0.2	1.8 ± 0.3	1.9 ± 0.2	2.2 ± 0.4	2.6 ± 0.4
NT	8.6 ± 0.2	10.7 ± 0.2	16.6 ± 0.5	20.9 ± 0.3	27.9 ± 0.6	36.9 ± 3.2