Thermodynamics of IP3 and adenophostin A binding to the NT and
IBC
From the effects of temperature on IP3 and adenophostin A binding
to the IBC and NT in CLM (Table 3,
Fig. 4, and Supplemental Fig. 6),
ΔG was determined
(ΔG = R ·
T lnKD) and thereby
ΔH and ΔS (eq. 3, assuming
ΔC = 0). A similar analysis in which
ΔC was not fixed to 0 provided
indistinguishable results (Supplemental Table 1).
−TΔS is also shown for 296 K. Results are means
± S.E.M., from three separate van't Hoff plots.