Fig. 6.
Bar graphs show the inhibitory effects of 3′,5′-cAMP (10 μM) and FOR (10 μM) on PDGF-BB (25 ng/ml)-induced DNA synthesis ([3H]thymidine incorporation) (top), collagen synthesis ([3H]proline incorporation) (middle), and cell number (bottom) in rat GMCs treated with 0.5 μM antisense, sense, or scrambled oligonucleotides to adenosine A2B receptor. Representative Western blot depicts the down-regulation of A2B receptor expression by antisense (AS), but not by sense (S) and scrambled (SC), oligonucleotides. Treatment with AS down-regulated A2B by 88 ± 6% (mean ± S.E.M.; n = 3). Values for growth studies represent mean ± S.E.M. from four separate experiments, each conducted in quadruplicate.