Fig. 7.

Representative gel images (of four experiments) demonstrating DNA laddering as an index of DNA fragmentation in the kidney tissues from sham-operated A₁WT (A₁WT sham) and A₁KO mice (A₁KO sham), A₁WT (A₁WT hepatic IR) or A₁KO mice (A₁KO hepatic IR) subjected to 60 min of hepatic ischemia and 24 h of reperfusion, and A₁WT mice pretreated with 0.4 mg/kg DPCPX (A₁WT hepatic IR+DPCPX) or 0.1 mg/kg CCPA (A₁WT hepatic IR+CCPA) and subjected to 60 min of hepatic ischemia and 24 h of reperfusion. Apoptotic DNA fragments were extracted according to the methods of Herrmann et al. (1994). This method of DNA extraction selectively isolates apoptotic, fragmented DNA and leaves behind the intact chromatin.