Skip to main content
. 2010 Jun;333(3):736–747. doi: 10.1124/jpet.110.166884

TABLE 1.

RT-PCR primers used in this study

Gene Species Amplicon Size Primer Sequences (Sense/Antisense) Annealing Cycle Number
bp °C
GAPDH Mouse 450 5′-ACCACAGTCCATGCCATCAC-3′ 65 15
5′-CACCACCCTGTTGCTGTAGCC-3′
A1AR Mouse/human 340 5′-CATTGGGCCACAGACCTACT-3′ 60 22
5′-GAAGTAGACCATGTACTCCA-3′
TNF-α Mouse 290 5′-TACTGAACTTCGGGGTGATTGGTCC-3′ 65 24
5′-CAGCCTTGTCCCTTGAAGAGAACC-3′
ICAM-1 Mouse 409 5′-TGTTTCCTGCCTCTGAAGC-3′ 60 21
5′-CTTCGTTTGTGATCCTCCG-3′
KC Mouse 202 5′-CAATGAGCTGCGCTGTCAGTG-3′ 60 26
5′-CTTGGGGACACCTTTTAGCATC-3′
MCP-1 Mouse 312 5′-ACCTGCTGCTACTCATTCAC-3′ 60 22
5′-TTGAGGTGGTTGTGGAAAAG-3′
MIP-2 Mouse 282 5′-CCAAGGGTTGACTTCAAGAAC-3′ 60 28
5′-AGCGAGGCACATCAGGTACG-3′