Figure 2.
Combined mutagenesis of charged residues in MiRP2 and KCNQ1 S4. A) Exemplar current traces recorded in oocytes coexpressing wild-type (wt) and mutant KCNQ1 and MiRP2 as indicated, recorded by TEVC using the protocol shown. Dashed line indicates 0 current level. B) Mean normalized conductance (G/Gmax, measured at arrow in A); for wild-type, D54A-MiRP2 and D55A-MiRP2 with wild-type, D242A-KCNQ1 or R237A-KCNQ1 as indicated; n = 7–44. C) Mean ΔΔG° for the closed to open transition, calculated from tail G/Gmax plots as in B and for other KCNQ1 single mutants with wild-type, D54A-MiRP2, and D55A-MiRP2 (M2); n = 7–44.