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. 2010 Jun 2;5(6):e10925. doi: 10.1371/journal.pone.0010925

Figure 4. Silencing the cold-inducible Fst expression impairs chill coma recovery in act-GAL4-driven females.

Figure 4

(A) Expression of Fst mRNA in untreated (kept at 25°C) and recovering (2 h at 25°C after 12 h at 0°C) females. Expression levels of Fst are normalized against the housekeeping reference RpS20 and values are √1/x transformed (mean±CI; n = 3). The symbol (*) indicates when the level is significantly different in act-GAL4/UAS-Fst versus act-GAL4/+ females (t-test). (B) Comparison of temporal recovery curves in act-GAL4/UAS-Fst (squares) versus act-GAL4/+ (circles) females. Time to recover from chill coma was monitored in females recovering at 25°C after 12 h of cold stress at 0°C. Each dot represents the mean percentage (±SE); 45 females were tested per line. (C) Mortality rate in act-GAL4/UAS-Fst versus act-GAL4/+ females. Mortality was assessed in flies recovering for 24 h at 25°C after 12 h of cold stress at 0°C. Bars represents the percentage (±CI) derived from 150 females in each line. The symbol (*) indicates a significant difference between lines (Chi square test). (D) Climbing activity monitored in act-GAL4/UAS-Fst versus act-GAL4/+ females. Measurements were taken in recovering females after 2, 4, 6 and 8 h at 25°C following 12 h at 0°C. Flies were categorized as fit (fast climbing) or recovering (slow climbing) or injured (no climbing). The symbol (*) indicate significant differences between lines (Chi square test, n = 70).