Fig. 1.

Adenovirus-mediated full-length utrophin expression does not restore sarcolemmal nNOS. (A) Representative dystrophin and nNOS immunofluorescence staining photomicrographs from normal and mdx muscles. Some reverent fibers can restore nNOS (arrowhead) whereas others cannot (arrow). (B) Representative immunofluorescence staining photomicrographs at 2 months after mdx muscles were infected with gutted adenoviruses. Left panels are dystrophin and nNOS staining following human full-length dystrophin gutted adenovirus infection. Right panels are utrophin and nNOS staining following mouse full-length utrophin gutted adenovirus infection. gAd, gutted adenovirus; HDys, human dystrophin; Utr, utrophin. (C) Two examples of serial sections from full-length utrophin gutted adenovirus infected muscles stained with antibodies against utrophin (left panels) and α-syntrophin (right panels). Myofibers expressing high levels of utrophin at the sarcolemma also expressed α-syntrophin. Asterisks indicate the same myofiber in serial sections.