Fig. 3.

Androgen promotes microtubule regrowth by a mechanism requiring the androgen receptor. (A) siRNA targeting the androgen receptor (AR) inhibited microtubule regrowth. HFFs were transfected with either control or AR siRNA, serum starved, and then replated onto fibronectin in either CCM1, SF DMEM or SF DMEM plus androgen, with androgen added only during the 5 minute regrowth period. Values are normalized mean areas ± s.d. calculated from 150 cells per condition in each of three independent experiments. *Values of AR siRNA-treated cells that are statistically different from those of the control siRNA-treated cells (P<0.05). (B) The inhibition of AR expression in each experiment was confirmed by western blotting. Values are AR protein levels ± s.d. in cells treated with control or AR siRNA from the three independent experiments shown in A. Inset: representative western blot.