Table 4.
A comparison of the relative amounts of moa mtDNA to bacteria DNA in bone and eggshell using qPCR. (Primer sets used in this assay. Samples from Bell Hill and Hukanui are temporarily held by RNH; SPAR, Southern Pacific Archaeological Research.)
| CT values |
||||||
|---|---|---|---|---|---|---|
| specimen ID no. | excavation site | moa taxon | moa 12Sa | bacteria 16Sb | ΔC*T | mean ΔCT |
| bone | ||||||
| BH_B1 | Bell Hill | D. robustus | 25.64 | 18.62 | 7.02 | 10.38 |
| S39957 | Bell Hill | D. robustus | 25.58 | 18.43 | 7.15 | moa : bacteria 1 : 1333 |
| BH_B2 | Bell Hill | P. elephantopus | 26.53 | 17.23 | 9.3 | |
| SPAR 5 | Redcliffs | E. crassus | 33.60 | 16.11 | 17.50 | |
| SPAR 6 | Redcliffs | E. crassus | 31.81 | 19.67 | 12.14 | |
| SPAR 9 | Redcliffs | E. geranoides | 26.50 | 17.31 | 9.19 | |
| eggshell | ||||||
| PN.J21.L2 | Pounawea | D. robustus | 28.61 | 26.95 | 1.66 | 3.41 |
| PN.H19.SP2.L1 | Pounawea | D. robustus | 28.40 | 26.91 | 1.49 | moa : bacteria 1 : 10.6 |
| PN.H18.L1 | Pounawea | D. robustus | 30.53 | 27.51 | 3.02 | |
| SPAR 12 | Redcliffs | D. robustus | 32.54 | 24.50 | 8.04 | |
| H7a_E20 | Hukanui 7a | P. mappini | 28.44 | 23.27 | 5.17 | |
| HP_E45 | Hukanui Pool | A. didiformis | 23.82 | 22.74 | 1.08 | |
*p < 0.003 (paired Students t-test).
a12Sa/h; Cooper et al. (2001).
bBac16sqPCR_F/Bac16sqPCR_R (present study) CT, cycle threshold.