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. Author manuscript; available in PMC: 2010 Jun 2.
Published in final edited form as: Nat Struct Mol Biol. 2009 Oct 18;16(11):1195–1199. doi: 10.1038/nsmb.1677

Figure 3.

Figure 3

Selected TFZFs shows repression of γ-globin promoter expression with when fused to the repressor domain KRAB. HeLa cells were transfected with TFZFs fused to KRAB (#γA-KRAB, #γA-opt-KRAB, #γL-KRAB, #γK-KRAB and #γM-KRAB gg1-KRAB) and a luciferase reporter plasmid expressing firefly luciferase under the control of γ-globin and Renilla luciferase under the control of the β-globin promoter. Specific activation of the γ-globin promoter was calculated by normalizing to Renilla luciferase values. Error bars represent standard deviation from the mean. Normalized luciferase activity of the selected TFZFs was compared to that of cells transfected with the non-DNA binding negative control Ala-KRAB and the positive control gg1-KRAB. For Ala-KRAB vs #γA-KRAB, p= 0.00018; For Ala-KRAB vs #γA-opt-KRAB, p= 9.6E-05.