Figure 2. BAFF-R is expressed on non-cultured primary ALL cells.
(A-C) We gated for CD19+ CD10+ IgM- cells (pre-B cells) from bone marrow mononuclear cells of a (A) healthy donor, (B) an ALL patient and from (C) peripheral blood of ALL patient US.7R, then assessed the gated population for expression of BAFF-R, TACI and BCMA. Gray line, receptor antibody; black line, isotype control. (D) Western blot analysis for BAFF-R. A1, A2 and A5, unrelated Ph-positive ALLs; A3 and A4, unrelated Ph-negative ALLs. Ramos and Jurkat; positive and negative controls. Tubulin, loading control.