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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cancer Res. 2010 May 11;70(11):4402–4411. doi: 10.1158/0008-5472.CAN-09-2767

Figure. 3. BAF57 depletion does not affect hSWI/SNF nucleosome remodeling activity.

Figure. 3

(A) Schematic of the 571 bp mononucleosome containing a 601-nucleosome positioning sequence. (B) Mononucleosome remodeling assays. A two-fold titration series of intact (i.e., from HeLa-Ini1/LucKD cells) and BAF57-depleted (i.e., from HeLa-Ini1/BAF57KD cells) hSWI/SNF complexes was assessed for remodeling activity. The extent of HhaI cutting was assessed and quantified using native PAGE followed by autoradiographic imaging and phosphoimaging analysis. (C) Quantification of the mononucleosome remodeling assays like those shown in panel B. Each bar = mean + SEM, n = 3.